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Homer1a is a core brain molecular correlate of sleep loss and weight – How to Keep the Brain Awake? The Complex Molecular Pharmacogenetics of Wake Promotion

Gene expression differences in mice divergently selected for methamphetamine sensitivity.

William Murphy
Friday, August 14, 2020
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  • Brain Part 4 : —

  • Research Feed. Effects of circadian misalignment on sleep in mice.

  • Synaptic changes during sleep include a downscaling of weak synapses, a process that appears to be promoted by Homer1a.

  • Overcompensation of paradoxical sleep after deprivation in two C57 strains. Mouse brain gene expression changes after acute and chronic amphetamine.

  • The brain structures controlling sleep in mammals have turned out to be highly conserved. This way, the profound changes in gene expression due to extended waking per se Maret et al, are accounted for.

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High-frequency gamma electroencephalogram activity in association with sleep-wake states and spontaneous behaviors in the rat. Get PDF. Differential increase in the expression of heat shock protein family members during sleep deprivation and during sleep. Ribozymes are catalytic RNAs that interact with a variety of small molecule substrates and cofactors, the intracellular abundance of which are sensed by riboswitches that modulate transcription, RNA stability, or translation.

  • Llss Altered in Sleep Deprived Subjects To identify pathways altered in sleep deprivation, we took the two largest cohorts of significant genes, the sustained and transient patterns, and separated them by direction of change. YKP10A presents weak interactions at the dopamine transporter but no relevant interactions with serotonin or norepinephrine reuptake in receptor-binding studies Gordon et al,

  • REM sleep-dependent short-term and long-term hourglass processes in the ultradian organization and recovery of REM sleep in the rat. Wake-promoting drugs are widely used to treat excessive daytime sleepiness.

  • Epilepsia 43 Suppl 2 : 28— Oscillations in local field potentials of the primate motor cortex during voluntary movement.

  • A randomized study of solriamfetol for excessive sleepiness in narcolepsy.

Solriamfetol for the treatment of excessive daytime sleepiness associated with narcolepsy. This technology is being investigated to combat malaria by targeting specific stretches of vector DNA and editing the genome at precise locations. Maret S. Electroencephalogram EEG analysis during drug-induced wakefulness revealed a transient approximately 2 Hz slowing of theta oscillations and an increase in beta-2 Hz activity only after YKP10A. Changes in EEG power spectra and behavioral states in rats exposed to the acetylcholinesterase inhibitor chlorpyrifos and muscarinic agonist oxotremorine. Trending Feeds. Type de document Articles.

Lack of the alanine-serine-cysteine transporter 1 causes tremors, seizures, and early postnatal death in mice. Dopamine depletion increases iw power and coherence of beta-oscillations in the cerebral cortex and subthalamic nucleus of the awake rat. Background Citations. Parent-of-origin genetic background affects the transcriptional levels of circadian and neuronal plasticity genes following sleep loss. Individual 'fingerprints' in human sleep EEG topography opens in new tab.

To test this, each region of the Venn diagram Fig. Microarray results informed our decisions regarding selection of gene products for measurement at the protein level. Finally, C. Nevertheless, because our gene signature approach considered only those genes that changed the most and because we have shown that the most significant changes detected by microarray analysis are routinely confirmed by qRT-PCR Maret et al,we feel confident about the gene pathway we identified. Disconnecting neural circuits from sensory input may facilitate the massive restructuring of brain circuits as memories mature Interestingly, Chga chromogranin A upregulation has been reported to suppress presynaptic vesicular release components [80][81] and may, at least in part, play an upstream role in mRNA expression changes associated with the pre-synapse. Keywords: genetics, model organism, optogenetics, sleep, sleep deprivation.

References

Enzyme Evolution This feed focuses on molecular models of enzyme evolution and new approaches such as adaptive laboratory evolution to metabolic engineering of microorganisms. Macromolecule biosynthesis: a key function of sleep. Get PDF. Overcompensation of paradoxical sleep after deprivation in two C57 strains.

Here is the latest research on metabolic syndrome and type 2 diabetes mellitus. Quality control and assurance in functional near infrared spectroscopy fNIRS experimentation. A In situ hybridization with Homer1a and eGFP antisense riboprobes indicated that both are expressed in similar brain structures. Comparison of the effects of modafinil and sleep deprivation on sleep and cortical EEG spectra in mice. High-frequency components of the rat electrocorticogram are modulated by the vigilance states. Dopamine depletion increases the power and coherence of beta-oscillations in the cerebral cortex and subthalamic nucleus of the awake rat. Create Alert Alert.

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Gamma activity Hz was induced by all drugs in a drug- and genotype-dependent manner. Corrleate modulation of hippocampal long-term memory is mediated by mTOR signaling. Some features of the site may not work correctly. Negative versus positive allosteric modulation of metabotropic glutamate receptors mGluR5 : indices for potential pro-cognitive drug properties based on EEG network oscillations and sleep-wake organization in rats. Glial solute carrier transporters in Drosophila and mice. This technology is being investigated to combat malaria by targeting specific stretches of vector DNA and editing the genome at precise locations.

Prog Neuropsychopharmacol Biol Psychiatry 12 : — The enhanced gamma activity observed during drug-induced wakefulness might thus reflect heightened cognitive performance and, at least for modafinil, an increase in working memory has been demonstrated in mice Beracochea et al, ; Pierard et al, and rats Miller et al, ; Ward et al, Microarray results pointed to sleep pressure signaling and macromolecular synthesis disruptions in the hippocampal CA1 region. Time course of accumulated differences in sleep time between the drug YKP10A, d- amphetamine, and modafinil and saline conditions. OrellanaMarius C. Note: error bars plotted but obscured by symbols. View Article Google Scholar 5.

INTRODUCTION

Sleep is regulated by a homeostatic process that determines its need and by a circadian process that determines its timing. Get PDF. Olfactory Navigation This feed focuses on the impact of smell and space perception, as well as mechanisms that underlie navigation behaviour in animals. The neuronal pathways involved in wake promotion are multiple and often not well characterized.

  • Interestingly, AP2 transcription factors are conserved regulators of sleep also in Drosophila and humans ,

  • Sexual dimorphism of the catechol-O-methyltransferase gene in narcolepsy is associated with response to modafinil.

  • YKP10A treatment produced a gene expression response resembling modafinil more than d- amphetamine. Note that the frequency range over which theta power is calculated moves as theta-peak frequency changes.

Four additional anchor screws ane implanted; one over the right hemisphere and three over the left hemisphere. Sleep 7: — Gene products were identified using literature and ontology database searches: 46 were significantly altered by sleep deprivation- the majority downregulated. J Sleep Res — A third cohort exposed to control or SD was used to validate transcriptional results with Western blots.

Forward genetic screening for sleep mutants identified a mouse mutant called Dreamlessa dominant mutation in a gene that encodes an cre channel required to control neural excitability, leading to a strong reduction of REM sleep but also causing defects in other rhythmic processes Stimulus-specific neuronal oscillations in orientation columns of cat visual cortex. Spectra were expressed relative to the EEG spectra of spontaneous wakefulness observed during the baseline light period Supplementary Figure S1. Journal of biology 8: 3. Introduction Age-related cognitive deficits are highly prevalent and constitute an important health risk in the human population reviewed in [1].

  • Theta power after drug administration was expressed as a percentage of that observed after saline injection.

  • This feed focuses on molecular models of enzyme evolution and new approaches such as adaptive laboratory evolution to metabolic engineering of microorganisms. Olfactory Navigation This feed focuses on the impact of smell and space perception, as well as mechanisms that underlie navigation behaviour in animals.

  • To gain insight into how wake-promoting compounds keep the brain awake, we emphasize first the spectral composition of the electroencephalogram EEG during drug-induced wakefulness, and second their effects on brain gene expression. Two sleep-related genes, Per2 and Homer1, were suppressed with age but upregulated with SD.

  • Launch Research Feed Feed. Homer1a is a core brain molecular correlate of sleep loss opens in new tab.

OrellanaMarius C. The overall finding of 4 genes agreeing, and two more agreeing in direction of change, particularly in a separate set of subjects measuring a different type of molecule, appears to constitute reasonable corroboration. To test this, we iteratively selected subsets of 21 arrays from the 53 used in the SD study and tested for significance. A systematic review of modafinil: potential clinical uses and mechanisms of action. Two sleep-related genes, Per2 and Homer1, were suppressed with age but upregulated with SD.

Enzyme Evolution This feed focuses on molecular models of enzyme evolution and new approaches such as adaptive laboratory evolution to metabolic engineering of microorganisms. Changes in EEG power spectra and behavioral states in rats exposed to the acetylcholinesterase inhibitor chlorpyrifos and muscarinic agonist oxotremorine. Metabolic syndrome is a complex disorder characterized by a cluster of metabolic abnormalities including hypertension, obesity, type 2 diabetes, and dyslipidemia. Figures and Topics from this paper. Investigational therapies for the treatment of narcolepsy.

Introduction

Chest 95— C Sensory stimulation during sleep increases the activity of the arousal system, suppressing sleep despite increased sleep drive. This article has been cited by other articles in PMC.

Four to 8 days of recovery from surgery was allowed before animals were connected to the recording leads. Drug effects on recovery of sleep were assessed as follows. It should be emphasized that the moleculzr changes in gene expression reported here were contrasted to the levels reached in mice that were injected with saline and then kept awake by gentle handling for the same duration. Takeuchi T, Harada E Age-related changes in sleep-wake rhythm in dog. Neurosci Lett : 43— The results from all iterations are plotted as a frequency histogram open circles. To test this, each region of the Venn diagram Fig.

Stimulus-specific neuronal oscillations in orientation columns of cat visual cortex. Maret S. This technology is being investigated to combat malaria by targeting specific stretches of vector DNA and editing the genome at precise locations. Negative versus positive allosteric modulation of metabotropic glutamate receptors mGluR5 : indices for potential pro-cognitive drug properties based on EEG network oscillations and sleep-wake organization in rats. Homeostatic and circadian contribution to EEG and molecular state variables of sleep regulation. The neuronal pathways involved in wake promotion are multiple and often not well characterized. Addiction-related gene regulation: risks of exposure to cognitive enhancers vs.

Sur les mêmes sujets

Schmidt MH The energy allocation function of sleep: a unifying theory of sleep, torpor, and continuous wakefulness. Neuron 88 : 20—32 [ PubMed ] [ Google Scholar ]. Data were subjected to bioinformatic analysis and contrasted with results from prior transcriptional profiling studies as noted in Results. During the last 1—1. Irwin MR Why sleep is important for health: a psychoneuroimmunology perspective.

  • In contrast, after d- amphetamine, an immediate increase in EMG was observed, whereas after modafinil no change or a reduction in EMG levels could be seen.

  • Sexual dimorphism of the catechol-O-methyltransferase gene in narcolepsy is associated with response to modafinil. High-frequency gamma electroencephalogram activity in association with sleep-wake states and spontaneous behaviors in the rat.

  • An association study of the brain-derived neurotrophic factor Val66Met polymorphism and amphetamine response.

  • Pradervand B.

  • Future studies examining the influence of stress, stress hormone, and their interaction with sleep and Homer1 expression with age may help to further clarify these issues. Of the many significant drug and strain differences we observed, only the most salient differences will be discussed below.

Ribozymes are catalytic RNAs that interact with a variety of small molecule substrates and cofactors, the intracellular abundance of which are sensed by riboswitches that modulate transcription, RNA stability, skeep translation. Riboswitches and Ribozymes Ribozymes are catalytic RNAs that interact with a variety of small molecule substrates and cofactors, the intracellular abundance of which are sensed by riboswitches that modulate transcription, RNA stability, or translation. Brain transcriptome and clustering analyses indicated that the three drugs have both common and specific molecular signatures. View 4 excerpts, cites methods and background.

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Differential increase in the expression of heat shock protein family members during sleep deprivation and during sleep. The European Journal of Neuroscience. Gamma activity Hz was induced by all drugs in a drug- and genotype-dependent manner. Exploratory behavior, cortical BDNF expression, and sleep homeostasis. Hagenbuchle O'Hara B.

Interestingly, Chga chromogranin A upregulation has been reported to suppress presynaptic vesicular release components [80][81] and may, at least in part, play an upstream role in mRNA expression changes associated with the pre-synapse. Dopaminergic-adrenergic interactions in the wake promoting mechanism of modafinil. Enhancement of learning processes following an acute modafinil injection in mice. These results are largely consistent with prior work on SD and interpretations are presented in Discussion. Psychostimulants affect the expression of immediate early genes, such as Fos, Arcand Homer1athe most Berke and Hyman, ; Yano and Steiner, The Journal of neuroscience : the official journal of the Society for Neuroscience 9: —

PubMed Google Scholar. Genes and neurons that control sleep may have functions that overlap with other processes. Thus, all template-identified genes are occurring at a significantly greater frequency than expected by chance. Acknowledgments We acknowledge Michael Bridges for contributions to behavioral studies, Philip Landfield for experimental design advice, and Christopher Norris for critical reading of the manuscript. Plasticity involves alterations in the size and composition of synapses.

Olfactory Navigation This feed focuses on the impact of smell and space correltae, as well as mechanisms that underlie navigation behaviour in animals. Trace amine-associated receptor 1 agonism promotes wakefulness without impairment of cognition in Cynomolgus macaques. Hagenbuchle and B. Skip to search form Skip to main content You are currently offline. The European Journal of Neuroscience. Riboswitches and Ribozymes Ribozymes are catalytic RNAs that interact with a variety of small molecule substrates and cofactors, the intracellular abundance of which are sensed by riboswitches that modulate transcription, RNA stability, or translation.

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Conversely, multiple studies have shown a decrease in Homer1 expression, with aging [31][32][33][68] suggesting that Homer1, and possibly other candidate sleep-pressure signaling systems, may serve as a lynch pin for discrepancies between young and brrain sleep behavior and molecular profiles. Forward genetic screening for sleep mutants identified a mouse mutant called Dreamlessa dominant mutation in a gene that encodes an ion channel required to control neural excitability, leading to a strong reduction of REM sleep but also causing defects in other rhythmic processes Perhaps the gentlest method for physical SD in zebrafish is through constant water flow Four experimenter-defined expression patterns templates were constructed to examine SD effects: sustained, transient, delayed, and linear Fig. Chest 95— Nature Reviews Disease Primers

Psychostimulants and epilepsy. Sharp wave-like activity in the hippocampus in vitro in mice lacking the gap junction protein connexin o Homer1a and 1bc levels in the rat somatosensory cortex vary with the time of day and sleep loss. By using sleep deprivation and transcriptome profiling in inbred mouse strains, we show that genetic background affects susceptibility to sleep loss at the transcriptional level in a tissue-dependent manner. Here is the latest research on olfactory navigation. MaretS.

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To further quantify the spectral changes in the waking EEG, the following frequency ranges were defined: theta: 4. Stat Med 9: — High-frequency gamma oscillations and human brain mapping with electrocorticography. FosterVladyslav V.

A role for cryptochromes in sleep regulation. Here is the latest research. Effects on two nights of recovery sleep. Acute wake-promoting actions of JNJ, a novel, diamine-based H3 antagonist.

Changes in EEG power spectra and behavioral states in rats exposed to the acetylcholinesterase inhibitor chlorpyrifos and muscarinic agonist oxotremorine. Save to Library Save. Distinct features of seizures induced by cocaine and amphetamine analogs. A In situ hybridization with Homer1a and eGFP antisense riboprobes indicated that both are expressed in similar brain structures. Addiction, dopamine, and the molecular mechanisms of memory. Metabolic syndrome is a complex disorder characterized by a cluster of metabolic abnormalities including hypertension, obesity, type 2 diabetes, and dyslipidemia.

Discover papers, protocols, and more Computational analysis of gene regulation in animal sleep deprivation. Sharp wave-like activity in the homer1 in vitro in mice lacking the gap junction protein connexin Sexual dimorphism of the catechol-O-methyltransferase gene in narcolepsy is associated with response to modafinil. This technology is being investigated to combat malaria by targeting specific stretches of vector DNA and editing the genome at precise locations. Gurcel and S. Publication Type.

Par les mêmes auteurs

It is not well understood how the various sleep centers in Drosophila interact to cause sleep, but they snd act, at least in part, in parallel pathways. On the other extreme, some animals such as bats sleep up to 20 h per day For new memories to form, specific synapses need to strengthen and new synapses need to form whereas other synapses need to weaken or disappear.

Sleep, arousal, and stress are intimately linked across species, and hyperarousal caused by mental stress is the main cause of insomnia in humans 2. Transcriptome analysis of sleeping brains has found that an increased expression of genes required for plasticity and protein synthesis during sleep is required for memory formation, suggesting that sleep serves the expression of plasticity genes to support learning 4243 This removal may involve neuronal shrinkage increasing the flux of interstitial fluid Topography of cocaine-induced gene regulation in the rat striatum: relationship to cortical inputs and role of behavioural context. CNS Spectr 45— Caenorhabditis elegans shows sleeping behavior across many life stages and conditions.

OllilaFabio PizzaPoul J. Both bootstrap and bias-corrected probabilities were computed to assess cluster—branch strength. Gene ie, probe sets clustering identified five main patterns of change in gene expression Figure 6b ; Supplementary Table S Also, it is not clear for which initial functions sleep has been selected for.

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Metabolic syndrome is a complex disorder yomer1a by a cluster of metabolic abnormalities including hypertension, obesity, type 2 diabetes, and dyslipidemia. Solriamfetol for the treatment of excessive daytime sleepiness associated with narcolepsy. Sleep is regulated by a homeostatic process that determines its need and by a circadian process that determines its timing.

By clicking accept or continuing to use the site, you agree to the terms outlined in our Privacy PolicyTerms of Serviceand Dataset License. Related Papers. View 3 excerpts, cites background and methods. This technology is being investigated to combat malaria by targeting specific stretches of vector DNA and editing the genome at precise locations.

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Effects of circadian misalignment on sleep in mice. Characterization of pharmacological and wake-promoting properties of the dopaminergic stimulant sydnocarb in rats. View 4 excerpts, cites methods and background. Modafinil, d-amphetamine and placebo during 64 hours of sustained mental work. Franken and M. Hagenbuchle and B.

Published under the terms of the CC BY 4. To quantify changes in Corfelate, calculated as signal variance, values were similarly expressed relative to the baseline light period. Future studies examining the influence of stress, stress hormone, and their interaction with sleep and Homer1 expression with age may help to further clarify these issues. Genes are ordered as in the clustering analysis. Psychostimulants affect the expression of immediate early genes, such as Fos, Arcand Homer1athe most Berke and Hyman, ; Yano and Steiner,

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Gurcel and S. Sexual dimorphism of the catechol-O-methyltransferase gene in narcolepsy is associated with response to modafinil. Sleep deprivation effects on growth factor expression in neonatal rats: a potential role for BDNF in the mediation of delta power. View on NAS.

  • Restriction of sleep by VLPO lesion in rats has been used to study the role of sleep in metabolism.

  • Langue Anglais.

  • Transient; C. F rats were divided into control and sleep deprivation groups.

  • Association between A2a receptor gene polymorphisms and caffeine-induced anxiety. DOI:

  • Sleep modulating agents.

This way, the profound changes in gene expression due to extended waking per se Maret et al, are accounted for. Taken together, this broad range home1a findings underscores the notion that various pathways can be activated pharmacologically to maintain the brain awake. Acute SD by sensory deprivation and chronic genetic SD are obviously different experiments and can lead to different conclusions as to the functions of sleep. For each drug treatment and each strain nine conditionsdifferentially expressed features compared with sleep-deprived control three chips vs three chips were identified using Limma Smyth, Perhaps the gentlest method for physical SD in zebrafish is through constant water flow To test this, we controlled for the lower statistical power of the NES study Fig.

Rather than merely conserving energy, sleep appears to slow down the progression of aging phenotypes Hasan, S. Clusters 1 and 5 showed the most interesting patterns, with opposite effects of YKP10A and d- amphetamine. The functions of sleep have been studied for decades, mostly by either correlation or SD induced by sensory stimulation. Complete SD in rodents caused weight loss, skin ulceration, sepsis, and ultimately death in experimental animals Browse Subject Areas?

Here is the latest research. Dopamine transporter gene associated with diminished subjective response to amphetamine. The effect of repeated amphetamine and cocaine administration on adrenal, gonadal and thyroid hormone levels in the rat plasma. A single-site, double-blind, placebo-controlled, dose-ranging study of YKP10A--a putative, new antidepressant.

Topography of cocaine-induced gene regulation in the rat striatum: relationship to cortical inputs and role of behavioural context. Supplementary information. Sleep deprivation reveals sleep functions Most of the theories regarding the functions of sleep are based on observations of processes that correlate with sleep, and causality is established by studying the consequences of sleep deprivation. For example, circadian rhythms control global physiology, and their abrogation can also result in sleep loss 61 ,

Here is the latest research on metabolic syndrome and type 2 diabetes mellitus. A In situ hybridization with Sleepp and eGFP antisense riboprobes indicated that both are expressed in similar brain structures. Strain differences in amphetamine sensitivity in mice. However, only remain rhythmic when mice are sleep-deprived at four time points around the clock, suggesting that most diurnal changes in gene transcription are, in fact, sleep-wake-dependent. Individual 'fingerprints' in human sleep EEG topography opens in new tab.

  • While the broad detrimental consequences of sleep loss are obvious, there still is no consensus as to what the direct consequences of sleep loss are, and how sleep carries out its functions at the molecular level.

  • Brainstem-diencephalo-septohippocampal systems controlling the theta rhythm of the hippocampus.

  • A systematic review of modafinil: potential clinical uses and mechanisms of action.

  • Olfactory Navigation This feed focuses on the impact of smell and space perception, as well as mechanisms that underlie navigation behaviour in animals.

  • Research Feed. Gurcel S.

Sleep deprivation reveals sleep functions Most of the theories regarding the functions of sleep are based on observations of processes that correlate with sleep, and causality is established by studying the consequences of sleep deprivation. Neuropsychopharmacology McEwen BS Plasticity of the hippocampus: adaptation to chronic stress and allostatic load. There are two principal approaches for triggering sleep loss via manipulations of brain areas that have been successfully applied in rodents.

Has PDF. The wake duration induced by YKP10A and d-amphetamine depended similarly on genotype, whereas opposite strain differences were observed after modafinil. Olfactory Navigation This feed focuses on the impact of smell and space perception, as well as mechanisms that underlie navigation behaviour in animals. Time-course gene expression analysis suggests that 2, brain transcripts are under circadian control.

Genetic sleep reduction has braiin associated with reduced lifespan in many but not all Drosophila sleep mutants. Individual genes listed in each condition or category, along with notes regarding putative function, are included below. This way, the profound changes in gene expression due to extended waking per se Maret et al, are accounted for. Transcriptome analysis of sleeping brains has found that an increased expression of genes required for plasticity and protein synthesis during sleep is required for memory formation, suggesting that sleep serves the expression of plasticity genes to support learning 4243 ,

Related Papers. Effects of chronic sleep fragmentation on wake-active neurons and the hypercapnic arousal response. Results Citations. Share This Paper. A non-circadian role for clock-genes in sleep homeostasis:a strain comparison.

We examined c-FOS induction in the brain to assess whether among the three drugs common neuronal pathways were implicated in wake promotion. We tested the prediction that the two treatments SD and NES would show relatively similar functional grouping results and that, if anything, SD would show a more refined dissection of functional groups identified in both SD and NES due to their similarity at the overlap level. Association between the casein kinase 1 epsilon gene region and subjective response to D -amphetamine. J Neurosci 21 : —

Parent-of-origin genetic background affects the transcriptional levels of circadian and neuronal plasticity genes following sleep loss. Homeostatic and circadian contribution to EEG and molecular state variables of sleep regulation. Sibah Hasan Paul Franken.

Alles GA Consistent with this idea, mutants exist that reduce sleep without causing hyperactivity see below. Neurosci Homdr1a : 89— However, manipulations of specific brain areas can lead to substantial sleep loss or gain Fig 4. In agreement with prior studies, body weight loss and corticosterone elevation Fig. Understanding sleep functions and the underlying mechanisms requires the study of sleeplessness and its consequences.

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The homeostatic regulation of sleep need is under genetic control. These results are largely consistent with prior work hoker1a SD and interpretations are presented in Discussion. BMC Neurosci 3 : Mignot E Why we sleep: the temporal organization of recovery. In the light periods, corresponding intervals were compared with respect to sleep onset; in the dark period with respect to dark onset.

Maret et al. Strain differences in amphetamine sensitivity in mice. Figures and Topics from this paper. The European Journal of Neuroscience. Modafinil more effectively induces wakefulness in orexin-null mice than in wild-type littermates opens in new tab.

Results Citations. Semantic Scholar Semantic Scholar's Logo. Modulation of recognition memory performance by light and its relationship with cortical EEG theta and gamma activities.

  • Other corroborating observations come from the increased c-FOS activity in the hippocampus of B6 mice, which was largest for the drug that also induced the largest increase in gamma activity; that is YKP10A.

  • Dorsaz and L.

  • Abstract Background Many aging changes seem similar to those elicited by sleep-deprivation and psychosocial stress.

  • Mouse brain gene expression changes after acute and chronic amphetamine. Discover the latest research here.

Methods Citations. A role for cryptochromes in sleep regulation. The correlation between specific EEG and gene-expression signatures suggests that the neuronal pathways activated to stay awake vary among drugs and genetic background. Sibah Hasan Paul Franken. Homer1a is a core brain molecular correlate of sleep loss opens in new tab. Homeostatic and circadian contribution to EEG and molecular state variables of sleep regulation.

Trace amine-associated receptor 1 agonism promotes wakefulness without impairment of cognition in Cynomolgus macaques. Acute cocaine-induced seizures: differential sensitivity of six inbred mouse strains. By clicking accept or continuing to use the site, you agree to the terms outlined in our Privacy PolicyTerms of Serviceand Dataset License. Dopamine depletion increases the power and coherence of beta-oscillations in the cerebral cortex and subthalamic nucleus of the awake rat. Event-related beta desynchronization in human subthalamic nucleus correlates with motor performance. Lack of the alanine-serine-cysteine transporter 1 causes tremors, seizures, and early postnatal death in mice.

Enhancement of learning processes following an acute modafinil injection in mice. View on NAS. Awakening properties of modafinil without paradoxical sleep rebound: comparative study with amphetamine in the rat.

You can also search for this author in PubMed Google Scholar. This supports the hypothesis that genetic SD without hyperactivity is possible in Drosophila Fig 4. Note that these ideas are overlapping. This example shows how optogenetics can be employed for functional studies of REM sleep 6.

Results Citations. Homeostatic and circadian contribution to EEG and molecular state variables of sleep regulation. Discover the latest research here. Modafinil, d-amphetamine and placebo during 64 hours of sustained mental work.

  • Synapse 49 : — Table S1.

  • Negative versus positive allosteric modulation of metabotropic glutamate receptors mGluR5 : indices for potential pro-cognitive drug properties based on EEG network oscillations and sleep-wake organization in rats.

  • The moderated t -statistics calculated by Limma were combined in one matrix with rows probe sets ordered according to the variance of the moderated t -statistics across the nine comparisons. Gene expression differences in mice divergently selected for methamphetamine sensitivity.

  • Bridging the gap between science and clinical practice.

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Pfister O. Association between A2a receptor gene polymorphisms and caffeine-induced anxiety. Pradervand B. Mouse brain gene expression changes after acute and chronic amphetamine. The effect of repeated amphetamine and cocaine administration on adrenal, gonadal and thyroid hormone levels in the rat plasma.

Skip to main content Thank you for visiting nature. A systematic review of modafinil: potential clinical uses and mechanisms of action. The EEG was subjected to a discrete Fourier transformation, yielding power spectra range: 0. This is best illustrated for the YKP10A experiments for which we observed profound changes in all four variables Figure 3. Sleep and torpor differ behaviorally as sleep is defined as a readily reversible state, whereas torpor generally is not rapidly reversible. Significant genes were further investigated with a post-hoc template matching strategy [32][45]. How to Keep the Brain Awake?

By generating a transgenic mouse line, we show that in Homer1-expressing cells specifically, apart from Homer1a, three other activity-induced genes Ptgs2, Jph3, and Nptx2 are overexpressed after sleep loss. Discover papers, protocols, and more DOI:

Intriguing parallels to work on other steroid hormones [69]suggest that, with age, the brain may shift its response to glucocorticoids. Zebrafish appear to have a quiet sleep state but evidence for a sleep state that resembles REM is lacking. This suggests that age-related changes interact in a more complex manner with SD-related genes. We observed that the conditions clustered according to drug treatment first, implying that the effect of drug on gene expression is stronger than the effect of genetic background.

First evidence of a delay-dependent working memory-enhancing effect of modafinil cote mice. Hochberg Y, Benjamini Y More powerful procedures for multiple significance testing. PubMed Google Scholar. Among the most significant effects in human studies was insomnia Amsterdam et al, and based on preliminary data, YKP10A was also shown to have wake-promoting action in rats A Ahnaou and W Drinkenburg; unpublished observations.

Major advantages of zebrafish as a sleep model are the high sleep loss of conservation of genes involved in sleep control, such as neuropeptide systems, a high level of conservation of key brain anatomical structures within a transparent brain, the possibility to model neuropsychiatric disorders as well as the possibility to scale up genetic and pharmacological screens 1314818283 Twenty four inverted clay flower pots served as elevated platforms 4 inch diameter within each chamber, allowing the animals to stay dry, move around, and access food and water supplied through the grated chamber lid. This process was repeated times with different randomly selected subsets of 21 chips, generating estimates of the number of significant genes expected if the SD study had only 21 chips. Figure 4. Interestingly, the increase in beta-2 activity after YKP10A administration greatly depended on genetic background and was absent in AK mice.

This example shows how optogenetics can be employed for functional studies of REM sleep 6. Conversely, multiple studies have shown a decrease in Homer1 expression, with aging [31][32][33][68] suggesting that Homer1, and possibly other candidate sleep-pressure signaling systems, may serve as a lynch pin for discrepancies between young and aged sleep behavior and molecular profiles. Scientific Reports We tested d- amphetamine, modafinil, and YKP10A, a novel wake-promoting compound, in three inbred strains of mice. The number of genes commonly regulated by the two experimental conditions was highly unlikely to have occurred by chance. Sleep appears to affect virtually all aspects of animal physiology, and numerous processes have been proposed to depend on the regular occurrence of sleep. Ground squirrels sleep during arousals from hibernation.

  • Four experimenter-defined expression patterns templates were constructed to examine SD effects: sustained, transient, delayed, and linear Fig. Neuroscience letters —

  • Olfactory Navigation This feed focuses on the impact of smell and space perception, as well as mechanisms that underlie navigation behaviour in animals. A randomized study of solriamfetol for excessive sleepiness in narcolepsy.

  • Drug effects on recovery of sleep were assessed as follows. Per2 is a circadian clock gene upregulated in prior SD studies [56] as well as in our NES treatment group, suggesting it may not be a purely SD-related finding.

Time-course gene expression analysis suggests that 2, brain molecluar are under… Expand. Sexual dimorphism of the catechol-O-methyltransferase gene in narcolepsy is associated with response to modafinil. Effects of chronic sleep fragmentation on wake-active neurons and the hypercapnic arousal response. The neuronal pathways involved in wake promotion are multiple and often not well characterized.

Tafti Published Biology, Medicine Proceedings of the National Academy of Sciences Sleep is regulated by a homeostatic process that determines its need and by a circadian process that determines its timing. Homer1a and 1bc levels in the rat somatosensory cortex vary with the time of day and sleep loss. The wake duration induced by YKP10A and d-amphetamine depended similarly on genotype, whereas opposite strain differences were observed after modafinil. Strain differences in amphetamine sensitivity in mice. By using sleep deprivation and transcriptome profiling in inbred mouse strains, we show that genetic background affects susceptibility to sleep loss at the transcriptional level in a tissue-dependent manner. Gurcel and S. Dopamine transporter gene associated with diminished subjective response to amphetamine.

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